The isolation of tubercle bacilli from resected lung lesions, III

Streptomycin sensitivity of tubercle bacilli from resected lungs.

Selective media in the isolation of tubercle bacilli from tissues.

Direct culture on Lowenstein-Jensen slopes and on three media made selective for tubercle bacilli by the addition of four antibacterial agents was compared with guinea-pig inoculation on 490 tissue specimens. Tubercle bacilli were obtained from 15 specimens by culture and 14 by guinea-pig inoculation; only one specimen was positive by guinea-pig and not by culture. The most efficient culture me...

Langhans' giant cells and tubercle bacilli in 91 pulmonary tuberculous lesions resected after prolonged combined chemotherapy.

The Langhans’ giant cell described in 1868 is a large cell with numerous nuclei arranged in a ring around the periphery of an eosinophilic cytoplasm.1’ 2 The cytoplasm is homogeneous or granular and may contain inclusions such as Mycobacterium tuberculosis. The foreign body type giant cell can be distinguished by the presence of nuclei throughout the substance of the cytoplasm rather than at th...

Streptomycin-dependent Tubercle Bacilli: a Simple Method for Isolation.

This report describes a simple method for the isolation of streptomycin-dependent tubercle bacilli from virulent cultures that have not previously been exposed to streptomycin. The in vivo isolation of streptomycin-dependent Mycobacterium tuberculosis has been reported by Spendlove et al. (1948) and Lenert and Hobby (1949). The in vitro procedure described here is applicable to the quantitative...

Cultivation of Tubercle Bacilli from Pleural Fluid

The diand tri-peptidase activity in white blood cells showed little variation from day to day (Table 11). This variation bore no relation to the changes in the number of white blood cells per c.mm. observed in cases of leukaemia. However, the activity of the enzymes differed in the diverse types ofwhite blood cells examined. White blood cells from cases of acute and chronic myeloid leukaemias g...